BioProBench

{"purpose":"Maintain hESCs in optimal conditions for growth and differentiation.","prior_step":"Preparation of vitronectin-coated plates","next_step":"Daily medium exchange"}

Maintain hESCs in Nutristem medium on 0.5u/c m^2 vitronectin N-precoated tissue culture-treated plastic in a humidified incubator (37^°C,10%C0_2).

Maintain hESCs in Nutristem medium on 0.5u/c m^2 vitronectin N-precoated tissue culture-treated plastic in a humidified incubator (37^°C,5%C0_2).

false

parameter

CO2 concentration is critical for maintaining pH balance in the medium.

TEST-ERR-000000

{"purpose":"Prepare medium for plating hESCs with ROCK inhibitor to enhance cell survival.","prior_step":"Preparation of vitronectin-coated wells","next_step":"Plating of thawed hESCs"}

Prepare enough Nutristem media for plating (3 mL per 3 cm well of a 6-well plate) with 1:100 ROCK inhibitor.

Prepare enough Nutristem media for plating (3 mL per 3 cm well of a 6-well plate) with 1:1000 ROCK inhibitor.

false

reagent

ROCK inhibitor concentration is crucial for preventing apoptosis during cell dissociation.

TEST-ERR-000001